anti lamb3 antibody  (Proteintech)

Journal: iMetaOmics

Article Title: Effects of SADS‐CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi‐omics investigation

doi: 10.1002/imo2.70015

Figure Lengend Snippet: Transcriptomic analyses of IPI‐2I cells after infection with the accessory protein deletion mutant viruses and the backbone virus. (A) Number of DEGs in each comparison group. Red indicates upregulation; blue indicates downregulation. Selection criteria are q value < 0.05 and |log 2 (fold change)| > 1. Volcano plots of DEGs in the (B) rSADS‐ΔNS3a infection group compared to the rSADS infection group and (C) rSADS‐ΔNS7 infection group compared to the rSADS infection group at 12 h postinfection. Significantly DEGs are color‐coded: red represents upregulated DEGs, blue indicates downregulated DEGs, while gray dots correspond to nonsignificant genes. GO enrichment analysis for the (D) rSADS‐ΔNS3a ‐12h versus rSADS ‐12h comparison group and (E) rSADS‐ΔNS7 ‐12h versus rSADS ‐12h comparison group. The 20 most significantly enriched GO terms (ranked by ascending p values) were visualized in a horizontal bar plot, with GO terms labeled on the y ‐axis and the −Log 10 ( p ‐value) of enrichment significance on the x ‐axis. Smaller p values (larger −Log 10 ( p ‐value) values) reflect greater enrichment significance. KEGG enrichment analysis for the (F) rSADS‐ΔNS3a ‐12h versus rSADS ‐12h comparison group and (G) rSADS‐ΔNS7 ‐12h versus rSADS ‐12h comparison group. The 20 most significantly enriched KEGG pathways (ranked by p ‐value) were visualized in a horizontal bar plot, with pathway labels on the y ‐axis and −Log 10 ( p ‐value) (representing enrichment significance) on the x ‐axis, where lower p values result in larger x ‐axis values denoting stronger statistical enrichment. (H) Heatmap of relative expression levels of major DEGs. Red indicates upregulation; blue indicates downregulation. (I) Bar graph of relative expression levels of the DEGs ARG1 , NAGS , PCK1 , LAMB3 , PLAU , and IL‐11 . hpi is the abbreviation for hours post infection. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: After the transfer was complete, the PVDF membrane was carefully removed and blocked in 5% skim milk; the membrane was then washed with Tris‐buffered saline containing Tween 20 (TBST) and incubated overnight at 4°C with polyclonal antibodies for NAGS (Proteintech, 21566‐1‐AP, 1:500); arginase‐1 (Proteintech, 16001‐1‐AP, 1:5000); PCK1 (Proteintech, 16754‐1‐AP, 1:5000); or LAMB3 (Proteintech, 26795‐1‐AP, 1:1000).

Techniques: Infection, Mutagenesis, Virus, Comparison, Selection, Labeling, Expressing

Journal: iMetaOmics

Article Title: Effects of SADS‐CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi‐omics investigation

doi: 10.1002/imo2.70015

Figure Lengend Snippet: Confirmation of mRNA and protein expression levels of DEGs in IPI‐2I cells after infection. (A) RT‐qPCR confirmation of mRNA levels of the DEGs ARG1 , NAGS , PCK1 , LAMB3 , PLAU , and IL‐11 . Gene expression levels were calculated using the 2 −ΔΔCt method, rSADS group was used as a control group, and β‐actin as an endogenous reference. (B) Western blot confirmation of protein expression levels of the DEGs ARG1, NAGS, PCK1, and LAMB3. All experiments were repeated at least three times.

Article Snippet: After the transfer was complete, the PVDF membrane was carefully removed and blocked in 5% skim milk; the membrane was then washed with Tris‐buffered saline containing Tween 20 (TBST) and incubated overnight at 4°C with polyclonal antibodies for NAGS (Proteintech, 21566‐1‐AP, 1:500); arginase‐1 (Proteintech, 16001‐1‐AP, 1:5000); PCK1 (Proteintech, 16754‐1‐AP, 1:5000); or LAMB3 (Proteintech, 26795‐1‐AP, 1:1000).

Techniques: Expressing, Infection, Quantitative RT-PCR, Gene Expression, Control, Western Blot

Journal: iMetaOmics

Article Title: Effects of SADS‐CoV accessory proteins NS3a, NS7a, and NS7b on viral pathogenicity: A multi‐omics investigation

doi: 10.1002/imo2.70015

Figure Lengend Snippet: Confirmation of mRNA transcription, protein expression levels, and metabolite levels after compensatory overexpression of proteins in mutated viruses. (A) RT‐qPCR confirmation of mRNA levels of DEGs ARG1 , NAGS , PCK1 , LAMB3 , PLAU , and IL‐11 post‐compensatory overexpression. Gene expression levels were calculated using the 2 −ΔΔCt method, Vector + rSADS group was used as a control group, and β‐actin as an endogenous reference. (B) Western blot confirmation of protein expression levels of DEGs ARG1, NAGS, PCK1, and LAMB3 post‐compensatory overexpression. All experiments were repeated at least three times. Heatmaps of relative expression levels of metabolites before and after (C) NS3a or (D) NS7a/NS7b compensatory overexpression. Red shading indicates upregulation, blue shading indicates downregulation, and red triangles indicate significant changes in relative expression levels of metabolites before and after compensation. AHHMO (C 10 H 15 N 3 O 5 ) is a custom abbreviation for 4‐amino‐1‐[4‐hydroxy‐5‐(hydroxymethyl)‐3‐methoxy‐2‐oxolanyl]‐2‐pyrimidinone.

Article Snippet: After the transfer was complete, the PVDF membrane was carefully removed and blocked in 5% skim milk; the membrane was then washed with Tris‐buffered saline containing Tween 20 (TBST) and incubated overnight at 4°C with polyclonal antibodies for NAGS (Proteintech, 21566‐1‐AP, 1:500); arginase‐1 (Proteintech, 16001‐1‐AP, 1:5000); PCK1 (Proteintech, 16754‐1‐AP, 1:5000); or LAMB3 (Proteintech, 26795‐1‐AP, 1:1000).

Techniques: Expressing, Over Expression, Quantitative RT-PCR, Gene Expression, Plasmid Preparation, Control, Western Blot

Journal: International journal of molecular sciences

Article Title: TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.

doi: 10.3390/ijms25168699

Figure Lengend Snippet: Figure 10. LN332-encoding genes’ transcripts are elevated and correlate with TNF in human carotid atherosclerotic lesions. Gene expression of LAMA3 (a), LAMB3 (b), and LAMC2 (c) in human carotid atherosclerotic tissues and adjacent macroscopically intact tissues (n = 32). Pearson’s correlation of LAMA3 (d), LAMB3 (e), and LAMC2 (f) with TNF in human carotid atherosclerotic lesions (n = 32). Solid line indicates Pearson’s correlation coefficient (r), and dashed line indicates 95% confidence band of the best-fit line. Data are acquired from human carotid atheroma gene expression (accession number, GSE43292). p-value smaller than 0.05 is considered statistically significant.

Article Snippet: AMAb91123, Atlas antibodies, Bromma, Sweden) Anti-LAMB3 (1:5000, catnr.

Techniques: Gene Expression